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Cell Quality Control

Remove low-quality cells based on morphology and intensity metrics.

QC Criteria

MetricDefault ThresholdDescription
Cell area50-5000 pxRemove debris and doublets
Nucleus ratio0.1-0.9Nuclear/cell area ratio
Total intensity>100Minimum signal
Autofluorescence<500Maximum background

CLI

celltype-refinery preprocess qc \
--input loaded/ \
--max-removal 0.15 \
--out output/filtered