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Fallopian Tube Example

Example annotation workflow for fallopian tube tissue.

Data​

  • 3.4M cells
  • 45 markers
  • 5 donors
  • 4 regions (fimbriae, ampulla, isthmus, uterine junction)

Workflow​

  1. Preprocessing with batch correction
  2. Leiden clustering at resolution 0.6
  3. Annotation with FT marker map
  4. Iterative refinement (3 rounds)
  5. Composition and spatial analysis

Results​

  • 91% assignment rate
  • 15 final cell types
  • Clear regional patterns